PHERAstar FSX

Powerful and most sensitive HTS plate reader

BMG LABTECH microplate reader PHERAstar FSX from the front view

The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV / vis spectrometer, and dedicated AlphaScreen ® and TRF lasers , the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.

Microplate reader technologies

More about our technologies

Alpha Technology Laser

Excitation lasers significantly improve performance and lower limits of detection, since they yield higher excitation energy at a specific wavelength when compared to xenon lamps.

Both the CLARIOstar and the PHERAstar FSX can be equipped with a laser dedicated to Alpha Technology (AlphaScreen ® , AlphaLISA ® and AlphaPlex TM ) detection. This dedicated laser outperforms any xenon-based light source by specifically exciting donors at 680 nm.

Graph showing the performance of direct optic bottom reading with the PHERAstar FSX multi-mode microplate reader

Direct optic bottom reading

Fluorescent and luminescent detection of adherent cells substantially profits from measurements performed from the bottom of the well. The PHERAstar FSX and the CLARIOstar have a free air optical path to the bottom of the microplate, improving sensitivity and eliminating the need for fiber optics or light guides .


Unlike conventional microplate readers, all BMG LABTECH readers require no displacement or installation of any additional hardware when switching between top and bottom reading modes.

Open PHERAstar showing pumps for reagent injection

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent.
All BMG LABTECH readers, with the exception of the SPECTROstar Nano , can be equipped with up to two built-in reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Schematic description of a BMG LABTECH UV/vis spectrometer

UV / vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV / vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second / well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Schematic description of a Dual Emission module of the PHERAstar FSX microplate reader from BMG LABTECH

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.

Automation

Automation is a key feature in high-throughput screening. Several BMG LABTECH microplate readers have been successfully integrated into various robotic systems. Since all instruments have the same xy dimensions and plate out / in position, once automation has been designed for one BMG LABTECH reader , the solution will work for all. All plate readers can be controlled through ActiveX or DDE interfaces. Moreover, the ASCII data output is the most versatile in the industry and works with most LIMS and automation platforms.

Unique TRF decay curve monitoring feature

Decay Curve Monitoring

A unique feature of the PHERAstar FSX , Decay Curve Monitoring provides an indispensable tool for assay troubleshooting and performance fine-tuning for TRF, TR-FRET and AlphaScreen ® assays. Thanks to special detectors with direct photon measurement system, fluorophore decay curves can be collected for one or two wavelengths with a user-selectable resolution of down to 2 µs.


While other TR-FRET microplate readers simply give an integration value for the area under the curve, the PHERAstar FSX gives much more!

Two optic modules

Optic modules

PHERAstar Optic Modules free users from worrying about which filter is installed, which dichroic is being used, or if the components are installed correctly. Optic Modules incorporate all the parts necessary to run a specific assay. They are labelled with a barcode and detected by the plate reader, eliminating the possibility of using the wrong filter set. Just drop a module into the PHERAstar and read the assay. It is that simple.

Using the automatic pathlength correction converts your microplate data to cuvette data for easy assay comparison and transfer

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and / or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Schematic description of incubation and shaking

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45 ° C or 65 ° C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

PHERAstar FSX

  • Optional Accessories

For more performance: Accessories for PHERAstar FSX

  • Microplate reader accessories LVis plate from the front view

    LVis Plate

    Low-volume measurements
  • Microplate stacker directed to the left with CLARIOstar in the background

    Microplate Stacker

    Microplate handling system
  • Microplate reader accessories evaluation plate from the front view

    Evaluation Plate

    Functional Microplate Reader Validation
  • Microplate reader accessories microplate shaker and incubator THERMOstar opened and from the front view

    THERMOstar

    Intelligent microplate incubation
  • Microplate Reader Accessories Filter

    Filters

    Filters for a wide range of applications

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