NanoBRET

Use a bright luciferase for bioluminescence resonance energy transfer to explore protein: protein interactions

Highly sensitive NanoBRET measurements using the LVF monochromator or filter-based readers

With the NanoLuc ® luciferase, Promega has created the next generation of BRET assays. The NanoLuc ® enzyme is smaller in size than other luciferases. At the same time its emission is brighter by several factors. These properties make the NanoLuc ® the perfect BRET donor . As BRET acceptor, a fluorescently labeled fusion protein are used such as the HaloTag ® that emits light in the red range (> 610 nm).

The NanoBRET assay technology is intended for studying protein-protein interactions in living cells. The proteins of major interest are membrane receptors such as G-protein coupled receptors (GPCR) and receptor tyrosine kinases (RTK).

All BMG LABTECH microplate readers can be equipped with optimized filters to record the two BRET signals coming from NanoLuc ® luciferase and fluorophore.

The CLARIOstar ® with its linear variable filter (LVF) monochromator ™ matches the two NanoBRET wavelengths without special filters. The flexibility to use the LVF monochromator for NanoBRET ™ measurements enables discrimination of donor of luminescence and two different acceptor fluorophores. This features a novel receptor internalization assay .

The boxes below lead on to NanoBRET related products.

BMG LABTECH microplate reader PHERAstar FSX from the front view

PHERAstar FSX

Powerful and most sensitive HTS plate reader
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BMG LABTECH microplate reader CLARIOstar Plus from the front view

CLARIOstar Plus

Most flexible Plate Reader for Assay Development
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The boxes below lead on to NanoBRET related application notes and further materials.

Synthesis of NanoBRET VEGF165

NanoBRET ™ assay quantitatively evaluates VEGF binding to the VEGFR2 in real-time in living cells

Microplate assay studies the receptor tyrosine kinase VEGFR2 in live cells and real-time using NanoBRET ™
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Schematic Description NanoBRET assay protein interactions

CRISPR / Cas9 genome-edited cells express nanoBRET-donor that monitors protein interaction and trafficking

Read how GPCRs are studied at endogenous expression levels by employing CRISPR / Cas9.
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Opened BMG LABTECH Application Notes binder

NanoBRET assay for monitoring of ligand binding to GPCRs in live cells, using the CLARIOstar and the PHERAstar FS

Read this application note example for NanoBRET
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Real-time profiling of receptor pharmacology

Get inspired how to use NanoBRET to monitor G-protein binding, receptor internalization or receptor heteromerization
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Enhanced protein-protein Interactions in living cells using the NanoBRET assay & CLARIOstar

Find out why the CLARIOstar's LVF monochromator is perfectly suited for NanoBRET measurements
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