Second Messenger Signaling

Detect multiple analytes in one well to increase your experimental outcome

Easily combine different fluorophores or detection modes in one experiment

State-of-the-art assays detect biological reactions at the site of action and in real-time. This can be accomplished by genetically encoded biosensors that report biological changes directly in the cell.


Montana Molecular developed fluorescent biosensors that not only detect GPCR-dependent changes in second messengers but also enable parallel monitoring of these. The sensors detect Gs- and Gi-related cyclic adenosine monophosphate (cAMP) as well as Gq-related diacylglycerol (DAG), phosphatidylinositol-biphosphate (PIP2) and calcium ions (Ca2 +). The sensors are available in red and green fluorescing versions to use them in a multiplexed assay.


The simplicity of combining different fluorophores in a kinetic measurement with BMG LABTECH instruments qualifies them to read real-time multiplex assays.

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Explore how the CLARIOstar ® detects second messenger responses to unambiguously identify specific GPCR-signaling.

BMG LABTECH microplate reader CLARIOstar Plus from the front view

CLARIOstar Plus

Most flexible Plate Reader for Assay Development
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Find below some further materials about second messengers.

Mechanism of cAMP assay

Real-time detection of Gs and Gi signaling in living cells

Learn how a genetically encoded cAMP sensor reports Gs and Gi simulation
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Multiplexed DAG and Ca2+ kinetics. Traces depict the average response to 30 μM Carbachol (n=16) expressed  as 100% response. R-GECO (red); green DAG (green). Carbachol was dispensed using on-board reagent injectors at the 30 second time point as indicated.

Simultaneous detection of GPCR second messengers in living cells

Real-time measurement of combined PIP2 or Ca2 + biosensor with DAG sensor in HEK293 cells
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