Read why our plate readers provide the perfect platform for measuring cellular metabolism.
Mitochondrial dysfunction is implicated in numerous disease states and is also a major mechanism of drug-induced toxicity. Oxygen consumption is one of the most informative and direct measures of mitochondrial function . Traditional methods of measuring oxygen consumption are hampered by the limitations of low throughput and high complexity.
The MitoXpress ® -Xtra assay from Agilent Technologies solves these limitations by allowing convenient, plate-based analysis of mitochondrial function. The assay employs MitoXpress ® , one of a family of phosphorescent oxygen sensitive probes developed by Agilent. This time-resolved fluorescence assay is based on the ability of O 2 to quench the excited state of the MitoXpress ® probe. As the test material respires (eg, isolated mitochondria , cell populations , small organisms , tissues and enzymes ), O 2 is depleted in the surrounding solution / environment , which is seen as an increase in probe signal. Changes in oxygen consumption reflecting changes in mitochondrial activity are seen as changes in MitoXpress ® probe signal over time.
The assay is non-chemical and reversible; a decrease in oxygen consumption (an increase in O 2 levels) is seen as a decrease in probe signal. MitoXpress ® can be analyzed using the Omega series fluorescence plate readers in Time Resolved Fluorescence mode, using standard 96- and 384-well microplates. The MitoXpress ® -Xtra assay combines the high data quality and information content of the oxygen electrode approach, with the throughput and convenience of microplate based assays. These capabilities make MitoXpress ® -Xtra the ideal tool for rapid compound screening, IC 50 generation and the application of structure-activity relationship approaches.
Discover our solutions for Agilent's Cell Metabolism Assays
Real-time measurement of intracellular O2 in mammalian cells
Measuring mitochondrial function and glycolytic flux in 3D cell cultures
Real-time monitoring of intracellular oxygen using MitoXpress-Intra
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