Ischemia Reperfusion Research

Mimic physiological and disease gas conditions with the CLARIOstar's atmospheric control unit

De- and re-oxygenation of plate readers' measurement chamber enables in vitro ischemia / reperfusion research

Oxygen supply is a critical factor adjudicating on health and disease. Rapid changes in oxygen occur if the blood flow is impaired in tissues. Although the blockage of blood vessels is associated with nutrient and oxygen deprivation (ischemia) and causes stroke and myocardial infarction, recovery of the blood stream (reperfusion) is even more deleterious as the rapid increase of oxygen induces reactive oxygen species and inflammation.

The Atmospheric Control Unit (ACU) of the CLARIOstar microplate reader was developed to support research on biological effects of oxygen dysregulation. It sustains hypoxic conditions, rapidly decreases oxygen to mimic ischemia and rapidly re-oxygenates the measurement chamber to simulate reperfusion. The course of oxygen and carbon dioxide concentrations is independently programmable by the user. The profile of oxygen deprivation and re-oxygenation is executed automatically by the ACU while the biological impact is recorded by the microplate reader.

The novel oxygen ramping feature enables in vitro studies of ischemia / reperfusion in high throughput and in real-time. Along with this unique feature, the CLARIOstar and its LVF Monochromator ™ offer high flexibility and sensitivity in all detection modes. The possibility to conveniently analyse multiple parameters in parallel (multiplexing) ensures the maximum output of each experiment.


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Find below some application note examples for ischemia / reperfusion research and the link to our products.

Microplate reader accessories Athmospheric Control Unit (ACU) from the front view

Atmospheric Control Unit

Enhanced cell-based assays
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Find below some application note examples for ischemia / reperfusion research.

Webinar intro image: Defining physiological normoxia

Defining physiological normoxia in cultured cells for translation to animal models and man

Learn how the CLARIOstar with ACU replicates in vivo oxygen conditions to increase the significance of your data
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A graph that shows the oxygen and carbon dioxide levels in the course of the experiment

Mitochondrial oxidant generation follows oxygen deprivation and re-oxygenation

Learn how rapid oxygen changes influences cellular respiration
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Example of ischemia-reperfusion atmospheric conditions

The CLARIOstar with ACU exposes cells to ischemia-reperfusion conditions and monitors their oxygenation

A redox-sensitive fluorophore measures oxidant generation in yeast colonies
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