Fluorescence intensity (incl. FRET)

Michael Herrwerth

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Fluorescence is the emission of light by a molecule (called fluorophore) that has been excited by light with a shorter wavelength than the emitted one. Fluorescence intensity detection is the measurement of this emitted light. A fluorescence plate reader consists of a light source, an excitation filter or monochromator, an emission filter or monochromator and a detector, usually a photomultiplier tube (PMT). As a result of a wavelength-specific excitation (light selected by the excitation filter / monochromator), the fluorophore emits light that is separated from the excitation light by the emission filter / monochromator and is measured by the PMT.

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Fluorescent assays

Fluorescence intensity is not an absolute measurement and is usually quantified in Relative Fluorescence Units (RFU). Thanks to the vast development of different fluorophores over the last decades, fluorescence intensity has become one of the most popular detection modes for plate readers, with thousands of assays available for many different applications.

Common applications for fluorescence intensity are calcium flux, DNA quantification , gene expression, protein interaction via Fluorescence Resonance Energy Transfer (FRET), enzymatic activities and more.

Application notes

Compatible readers

  • BMG LABTECH microplate reader CLARIOstar Plus from the front view

    CLARIOstar Plus

  • BMG LABTECH microplate reader PHERAstar FSX from the front view

    PHERAstar FSX

  • BMG LABTECH microplate reader POLARstar Omega from the front view

    POLARstar Omega

  • BMG LABTECH microplate reader FLUOstar Omega from the front view

    FLUOstar Omega

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