Cell metabolism

Measure glycolysis and respiration in live cells with a CLARIOstar

Determine extracellular acidification and oxygen consumption in real-time

The metabolic condition of a cell sheds light on its state of health. The rate of glycolysis is one metabolic factor impacting cellular physiology and pathology. For instance, cancer cells exhibit a higher rate of glycolysis compared to healthy counterparts. Since lactate is produced in the course of glycolysis and subsequently released into the extra-cellular space, it acidifies the surrounding medium. The so-called extracellular acidification is hence used to monitor glycolytic flux.

A second metabolic parameter is the consumption of molecular oxygen (O 2 ) as it reflects oxidative phosphorylation. In cancer cells this pathway of energy production is decreased. Furthermore changes in oxygen consumption may indicate mitotoxicity of drugs.

Agilent Technologies provides probes that are sensitive to either pH or O 2 and reliably trace both metabolic processes: glycolysis and respiration. Both probes can be used simultaneously, assuring maximal information output from your experiment.

Display of a cell

Discover our solutions for Agilent's Cell Metabolism Assays

Read why our plate readers provide the perfect platform for measuring cellular metabolism.

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The CLARIOstar microplate reader is the ideal tool to measure these metabolic assays as it easily detects the probes and can be equipped with an Atmospheric Control Unit (ACU). This accessory allows working at desired oxygen and carbon dioxide concentrations that resemble physiological conditions. It further enables to work at steady O 2 and CO 2 concentrations, a requirement for determination of extracellular acidification (ECA) and oxygen consumption rate (OCR).

Find below the link to our cell metabolism related product.

BMG LABTECH microplate reader CLARIOstar Plus from the front view

CLARIOstar Plus

Most flexible Plate Reader for Assay Development
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The boxes below lead on to cell metabolism application notes and webinars.

Mammalian cell

Real-time measurement of intracellular O2 in mammalian cells

CLARIOstar with ACU used to determine the effect of compound addition to the level of intracellular oxygen concentration.
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Oxygen consumption profiles from A549 RAFT cultures of increasing cell density

Measuring mitochondrial function and glycolytic flux in 3D cell cultures

Read this application note example for cell metabolism performed on a CLARIOstar microplate reader.
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